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RT-PCR was used to measure the gene expression (mRNA) of MMP1 (a metalloproteinase), MMP2, TIMP1, and TIMP2 (tissue inhibitors of metalloproteinases) in treated HDFa cells

4, Supplementary Table 2)

Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy

The most abundant proteoglycan in the NP is aggrecan, but it should also be noted that decorin, biglycan, fibromodulin, and versican have also been observed (Sztrolovics et al., 1999